Signalling

Part:BBa_K1343019

Designed by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon   Group: iGEM14_Technion-Israel   (2014-10-06)

Plux->Green Renilla Luciferase->tetR

The promoter Plux is induced by the binding of an AHL-luxR dimer. This bio-brick leads to the synthesis of the enzyme Green Renilla Luciferase, which catalyses a luminescent reaction. TetR can be used to repress the promoter Ptet.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal XhoI site found at 57
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 188
    Illegal AgeI site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None